| PL |
Name |
Time |
Wind |
H# |
|
| 1 | Ashlan Best Speed River | 23.88Q | 1.5 | 6 | |
| 2 | Alyssa Marsh Durham Legion | 24.20Q | 1.5 | 2 | |
| 3 | Zoe Sherar Speed River | 24.37Q | 1.6 | 4 | |
| 4 | Kendra Leger Speed River | 24.71Q | 0.3 | 1 | |
| 5 | Skyy Diop The Gazelles | 25.10Q | 1.2 | 5 | |
| 6 | Alanah Broomfield I Be Fast | 25.20Q | 0.7 | 3 | 25.195 |
| 7 | Tomi Bamigbade Extreme Velocity | 25.04q | 0.3 | 1 | |
| 8 | Diana Boa York University | 25.19q | 1.2 | 5 | |
| 9 | Allysia Parry Speed Academy | 25.20q | 1.5 | 2 | 25.194 |
| 10 | Amy Stieh Lakehead Running | 25.55q | 0.7 | 3 | |
| 11 | Julia Vanhoucke St Thomas Legion | 25.69q | 1.5 | 2 | |
| 12 | Milenne Habash Speed River | 25.74q | 0.7 | 3 | |
| 13 | Jazmyn Lewis Speed Academy | 25.89q | 0.7 | 3 | |
| 14 | Kayla Vieux C.A.N.I. Athletics | 25.93q | 1.5 | 6 | |
| 15 | Sandrine Desrosiers Corsaire-Chaparal | 25.95q | 1.2 | 5 | |
| 16 | Amelia Matthews Laurel Creek T.F.C. | 26.14q | 0.7 | 3 | |
| 17 | Tia Lampo Lakehead Running | 26.15 | 0.3 | 1 | |
| 18 | Elizabeth Klosa Laurel Creek T.F.C. | 26.18 | 1.6 | 4 | |
| 19 | Kassidy Akposi Project Athletic | 26.23 | 1.6 | 4 | |
| 20 | Aliyah Broomfield I Be Fast | 26.27 | 1.5 | 6 | |
| 21 | Paulina Procyk Ottawa Lions T.F.C. | 26.38 | 1.5 | 2 | |
| 22 | Kenna Hopkins Burlington | 26.47 | 0.3 | 1 | |
| 23 | Angelisa Wallace Terminal Velocit | 26.49 | 0.3 | 1 | |
| 24 | Doyin Ogunremi Ottawa Lions T.F.C. | 26.55 | 1.6 | 4 | |
| 25 | Madison Smodis Lakehead Running | 26.58 | 1.5 | 6 | |
| 26 | Elise MacDonald Laurel Creek T.F.C. | 26.67 | 1.5 | 6 | |
| 27 | Kaysen Drummond Terminal Velocit | 26.71 | 1.2 | 5 | |
| 28 | Madison Newman Lakehead Running | 26.78 | 0.7 | 3 | |
| 29 | Zahmoya Grant-Bingling South Simcoe | 26.91 | 1.2 | 5 | |
| 30 | Juliette Nadeau Corsaire-Chaparal | 26.99 | 1.6 | 4 | |
| 31 | Gemiah Lewis Terminal Velocit | 27.00 | 1.5 | 2 | |
| 32 | Kimora Harris U Windsor AC | 27.12 | 0.3 | 1 | |
| 33 | Tessa O'Brien Hamilton Olympic | 27.13 | 1.5 | 6 | |
| 34 | Cassidy Kent London Western | 27.14 | 1.5 | 2 | 27.139 |
| 34 | Zyeisha Grant-Bingling South Simcoe | 27.14 | 1.5 | 2 | 27.139 |
| 36 | Bethany Suen York University | 27.18 | 1.6 | 4 | |
| 37 | Lucy Hempstead C.A.N.I. Athletics | 27.75 | 0.7 | 3 | |
| 38 | Britney Hawker Terminal Velocit | 27.86 | 0.3 | 1 | |
| 39 | Marissa Papaconstantinou U of Toronto TC | 28.16 | 1.2 | 5 | |
| 40 | Victoria Khounborinh York Region | 28.47 | 1.5 | 6 | |
| 41 | Kaitlyn Effenberger Ottawa Lions T.F.C. | 28.99 | 1.2 | 5 | |