| PL |
Name |
Time |
H# |
|
| 1 | Will McBride Unattached Ontario | 48.17Q | 4 | |
| 2 | Alanzo Ryan U of Toronto TC | 49.28Q | 3 | |
| 3 | Nicolas Morales Speed Academy | 49.58Q | 2 | |
| 4 | Marcus Nandlal U of Toronto TC | 50.40Q | 1 | |
| 5 | Daniel Burke Ultimate Body | 49.95q | 3 | |
| 6 | Matthew Hebert Ottawa Lions T.F.C. | 50.18q | 3 | |
| 7 | Aidan Vanbeek C.A.N.I. Athletics | 50.38q | 4 | |
| 8 | Kyron Maloney The Gazelles | 50.43q | 1 | |
| 9 | Liam Rivard Central Toronto | 50.59 | 1 | |
| 10 | Jemini Archer Project Athletic | 51.14 | 2 | |
| 11 | Joe Mark Central Toronto | 51.52 | 1 | |
| 12 | Gabriel Samson Rive-Sud | 51.59 | 2 | |
| 13 | Alex Pope C.A.N.I. Athletics | 51.63 | 3 | |
| 14 | Akot Aken U Windsor AC | 51.95 | 1 | |
| 15 | Akil Julien U of Toronto TC | 52.50 | 4 | |
| 16 | Cornelius Liburd Ottawa Lions T.F.C. | 53.10 | 4 | |
| 17 | Jordan Palmer St Thomas Legion | 53.10 | 4 | |
| 18 | Matheus Chaves Central Toronto | 53.29 | 3 | |
| 19 | Cameron McLeod Hamilton Olympic | 53.37 | 2 | |
| 20 | Dan Kazumba Strive Athletics | 53.50 | 3 | |
| 21 | Alexander Collins C.A.N.I. Athletics | 53.60 | 1 | |
| 22 | Liam Ford Hamilton Olympic | 53.86 | 3 | |
| 23 | Jacob Thompson C.A.N.I. Athletics | 53.99 | 2 | |
| 24 | Cole Hines U Windsor AC | 54.74 | 2 | |
| 25 | Josiha Kerr Brampton Track | 55.38 | 1 | |
| 26 | Cole Kapitain Central Toronto | 55.57 | 4 | |
| 27 | Ken Lorbetskie Ottawa Lions T.F.C. | 55.71 | 1 | |
| 28 | Jonathon Ross Central Toronto | 56.50 | 2 | |
| 29 | Jaxon Croth Central Toronto | 56.66 | 2 | |
| 30 | Juan Pablo Medina York University | 58.70 | 4 | |
| 31 | Nicholas Pasquini York University | 1:00.93 | 3 | |