| PL |
Name |
Time |
Wind |
H# |
|
| 1 | Trinity Miller 03 The Gazelles | 24.20 | -0.1 | 7 | 10 |
| 2 | Jillian Catton 03 Brampton Track | 24.48 | -0.1 | 7 | |
| 3 | Nicole McKenzie 03 Boost Athletics | 24.80 | -0.1 | 7 | |
| 4 | Ashantai Bollers 02 Project Ath. | 24.93 | -0.1 | 7 | |
| 5 | Ivana Atem 02 Flying Angels | 25.45 | -0.7 | 6 | |
| 6 | Madison St. Louis 03 Durham Legion | 25.54 | -1.4 | 5 | |
| 7 | Zahnee Grant-Bingling 03 South Simcoe | 25.70 | -0.1 | 7 | |
| 8 | Jasmine Kidd 03 The Gazelles | 25.76 | -0.1 | 7 | |
| 9 | Collette Wolfe 02 Invictus AC | 25.81 | -0.1 | 7 | |
| 10 | Avery Ferron 03 Burlington T.F.C | 26.05 | -0.7 | 6 | |
| 11 | Chloe Schneeberg 03 The Gazelles | 26.19 | -0.7 | 6 | |
| 12 | Aliyah Logan 02 Speed Academy | 26.20 | -0.7 | 6 | |
| 13 | Hannah Adderley Julien 03 The Gazelles | 26.22 | -0.7 | 6 | |
| 14 | Franchery Carrion 03 Mississauga Oly. | 26.38 | -1.4 | 5 | |
| 15 | Rachel Watson 02 Hamilton Olympic | 26.44 | -0.7 | 6 | |
| 16 | Vanessa Lu Langley 02 Ottawa Lions T.F.C. | 26.55 | -0.7 | 6 | |
| 17 | Emily Fritzley 02 Speed River | 26.66 | -1.4 | 5 | |
| 18 | Jessica Bailey 03 Border City AC | 26.73 | -1.4 | 5 | |
| 19 | Emily Olesinski 03 The Gazelles | 27.01 | -1.4 | 5 | |
| 20 | Khalis Somerville 03 Speed Academy | 27.05 | -1.4 | 5 | |
| 21 | Titilayomi Sijuade 02 Mississauga Oly. | 27.06 | | | |
| 22 | Jaden Delima 02 Niagara Olympic | 27.12 | -1.0 | 1 | |
| 23 | Abigale Acheampong 03 Unattached Ontario | 27.25 | -1.0 | 1 | |
| 24 | Grace Manderson 03 Laurel Creek T.F.C. | 27.38 | -0.9 | 3 | |
| 25 | Jenae Hylton 03 Mississauga Oly. | 27.39 | -1.4 | 5 | |
| 26 | Nayla Brooks 02 Speed Academy | 27.41 | 0.7 | 2 | |
| 27 | Kalise Tyrell 03 Athletiques Int | 27.41 | 27.410 | | |
| 28 | Siena Sujitno 03 Uni. Toronto TC | 27.49 | | | |
| 29 | Taaliyah Davidson Baptis 02 Unattached Ontario 27.56 -1.0 1 |
| | | |
| 30 | Marissa McCutcheon 03 Ottawa Lions T.F.C. | 27.66 | | | |
| 31 | Priscilla Nyarkoh 03 The Gazelles | 27.71 | | | |
| 32 | Kseniya Poberezhnaya 03 Speed River | 27.77 | -1.4 | 5 | |
| 33 | Chiara Price 02 Uni. Toronto TC | 27.78 | 0.7 | 2 | |
| 34 | Sydney Baxter 03 The Gazelles | 27.88 | 0.7 | 2 | |
| 35 | Kyra Luke 03 Mississauga Oly. | 27.90 | | | |
| 36 | Hanna Schroeder 02 Border City AC | 27.94 | -0.9 | 3 | |
| 37 | Jenelle Lyman 02 Unattached Ontario | 27.96 | -0.9 | 3 | |
| 38 | Teju Oladipo 03 Thorold Elite | 28.01 | | | |
| 39 | Izirouwa Usuanlele 02 Thorold Elite | 28.12 | | | |
| 40 | Raine Garrell 03 The Gazelles | 28.15 | 0.7 | 2 | |
| 41 | Atrab Bardoun 03 Speed River | 28.19 | -0.9 | 3 | |
| 42 | Cassidy Gute 03 Laurel Creek T.F.C. | 28.64 | -0.9 | 3 | |
| 43 | Shayann Henry 03 Durham Legion | 28.65 | 0.7 | 2 | |
| 44 | Bailey Fuchs 03 Kingston Track | 28.67 | -0.9 | 3 | |
| 45 | Jordyn Crowder 03 Border City AC | 28.73 | 0.7 | 2 | |
| 46 | Earen Klassen 03 St Thomas Legion | 28.83 | -0.9 | 3 | |
| 47 | Katie Johnson 03 Boost Athletics | 29.10 | -0.9 | 3 | |
| 48 | Shenai Royal 02 Unattached Ontario | 29.75 | -1.0 | 1 | |
| 49 | Kimberly Uwalila-Olumese 03 The Gazelles 29.88 -1.0 1 |
| | | |
| 50 | Julia Kipfer-Portt 03 Etobicoke Track | 30.56 | 0.7 | 2 | |
| 51 | Jillian Christie 02 York Uni. T.F.C. | 30.83 | 0.7 | 2 | |