| PL |
Name |
Time |
H# |
|
| 1 | Jenna James
03 The Gazelles | 56.08 | 4 | |
| 2 | Nicole McKenzie
03 Boost Athletics | 56.35 | 4 | |
| 3 | Alena Ivanov
03 London Legion | 57.79 | 4 | |
| 4 | Cassandra Williamson
02 The Gazelles | 57.90 | 4 | |
| 5 | Kathryn Draves
03 South Simcoe | 59.27 | 4 | |
| 6 | Jaden Delima
02 Niagara Olympic | 59.29 | 2 | |
| 7 | Madison St. Louis
03 Durham Legion | 59.78 | 3 | |
| 8 | Kyra Vellinga
03 St Thomas Legion | 59.93 | 4 | |
| 9 | Andriana Erotocritou
03 The Gazelles | 59.94 | 4 | |
| 10 | Julia Bérubé Boutin
02 Uni. Toronto TC | 59.95 | 3 | |
| 11 | Sara Escallon
02 Uni. Toronto TC | 1:00.34 | 2 | |
| 12 | Pearl Vannooten
02 Brantford T.F.C. | 1:00.39 | 4 | |
| 13 | Jenelle Lyman
02 Unattached Ontario | 1:00.71 | 3 | |
| 14 | Emily Olesinski
03 The Gazelles | 1:00.87 | 3 | |
| 15 | Lara Watchorn
03 Flying Angels | 1:00.92 | 3 | |
| 16 | Daphne Tambakis
02 Blue Devils | 1:01.24 | 3 | |
| 17 | Khalis Somerville
03 Speed Academy | 1:01.25 | 2 | |
| 18 | Olateju Obisesan
02 Speed River | 1:01.42 | 2 | |
| 19 | Hannah Adderley Julien
03 The Gazelles | 1:02.58 | 3 | |
| 20 | Lauren Roy
02 Speed River | 1:02.88 | 1 | |
| 21 | Sophie Sun
02 Etobicoke Track | 1:03.65 | 3 | |
| 22 | Elaina Kischuk
03 York Uni. T.F.C. | 1:03.87 | 2 | |
| 23 | Makayla McKibbin
02 Border City AC | 1:04.25 | 1 | |
| 24 | Jordyn Crowder
03 Border City AC | 1:04.82 | 1 | |
| 25 | Emma Dinning
03 Thorold Elite | 1:05.08 | 1 | |
| 26 | Nayla Brooks
02 Speed Academy | 1:05.24 | 1 | |
| 27 | Stacie Morris
02 Project Ath. | 1:05.45 | 1 | |
| 28 | Earen Klassen
03 St Thomas Legion | 1:05.51 | 2 | |
| 29 | Marie Bérubé Boutin
03 Uni. Toronto TC | 1:07.22 | 2 | |
| 30 | Katie Johnson
03 Boost Athletics | 1:07.25 | 1 | |
